[Lactobacillus gasseri] Lactobacillus gasseri suppresses Th17 pro-inflammatory response and attenuates allergen-induced airway inflammation in a mouse model of allergic asthma

e 2083

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Lactobacillusgasseri suppresses Th17 pro-inflammatory response and attenuatesallergen-induced airway inflammation in a mouse model of allergic asthma

Ren-Long Jan,Kung-Chih Yeh, Miao-Hsi Hsieh, Yen-Lin Lin, Hui-Fang Kao, Pi-Hsueh Li,

Yuh-ShinChang and Jiu-Yao Wang

Department ofPediatrics, Chi-Mei Medical Center, Liou-Yin, Tainan, Taiwan, ROC; College ofMedicine, Institute of Clinical Medicine, National Cheng Kung UniversityHospital, Tainan, Taiwan, ROC; College of Medicine, Institute of Microbiologyand Immunology, National Cheng Kung University Hospital, Tainan, Taiwan, ROC; Collegeof Medicine, Institute of Basic Medical Sciences, National Cheng KungUniversity Hospital, Tainan, Taiwan, ROC; College of Medicine, Institute ofPhysiology, National Cheng Kung University Hospital, Tainan, Taiwan, ROC; 6Department of Pediatrics, College of Medicine, National Cheng Kung UniversityHospital, No. 138, Sheng Li-Road, Tainan 70428, Taiwan, ROC

Abstract

Probiotics are normal inhabitants of the gastrointestinal tract of man and are widely considered to exert a number of beneficial effects in many diseases. But the mechanism by which they modulate the immune system is poorly understood. The present study was planned to explore the anti-allergic effect of Lactobacillus gasseri on a mouse model of allergic asthma. Dermatophoides pteronyssinus (Der p) sensitised and challenged BALB/c mice were orally administered via oral administration with three different doses of L. gasseri (low, 1 X 10e6 colony-forming units (CFU); medium, 2 X 10e6 CFU; high, 4 X 10e6 CFU), in 700 ml of PBS daily, starting from 2 weeks before Der p sensitisation for 4 weeks. After the allergen challenge, airway responsiveness to methacholine, influx of inflammatory cells to the lung, and cytokine levels in bronchoalveolar lavage (BAL) fluids and splenocytes culture were assessed. Our results showed that oral administration of a high dose of L. gasseri (4 X 10e6 CFU) decreased airway responsiveness to methacholine, attenuated the influx of inflammatory cells to the airways and reduced the levels of TNF-a, thymus and activation-regulated chemokine (TARC) and IL-17A in BAL fluids of Der p-sensitised and -challenged mice. Moreover, L. gasseri decreased IL-17A production in transforming growth factor-a and IL-6 stimulated splenocytes and cell numbers of IL-17 producing alveolar macrophages in L. gasseri-treated mice as compared to non-treated, Der p-sensitised and -challenged mice. In conclusion, oral administration with L. gasseri can attenuate major characteristics of allergen-induced airway inflammation and IL-17 pro-inflammatory immune response in a mouse model of allergic asthma, which may have clinical implication in the preventive or therapeutic potential in allergic asthma.

제목

락토바실러스 가세리(Lactobacillus gasseri)는 알레르기성 천식의 마우스 모델에서Th17 염증 반응을 억제하고 알레르겐 유발 기도 염증을 약화시킨다.

내용

프로바이오틱스(Probiotics)는 인간 위장관에 거주하는 유익균으로, 다수의 질병에서 다양한 유익한 효과를 발휘하는 것으로 널리 간주된다. 그러나그들이 면역 체계를 조절하는 메커니즘은 잘 알려져 있지 않다. 본 연구는 알레르기성 천식의 마우스 모델에서락토바실러스 가세리(Lactobacillusgasseri)의 항 알레르기 효과를 탐구하기 위해 계획되었다. 데르마토피데스 페로니스시누스(Dermatophoides pteronyssinus; Der p)에 민감하고 항진성을 가진 BALB/c 쥐에게 매일 700 ml의 PBS를 Der p 감작(sensitization)2주 전부터 총 4주 간 L. gasseri를 세 가지 복용량(저용량, 1 × 10e6 CFU; 중간 용량, 2 × 10e6 CFU; 고용량 4 X 10e6 CFU)으로 나누어 경구 투여하였다. 알레르겐(알레르기 항원) 투여 후 메타콜린에 대한 기도의 반응, 염증 세포의 폐 유입, 기관지폐포세척액(BAL fluids) 및 비장 세포 배양에서의 사이토카인 수치를 측정하였다. 연구결과는 L. gasseri를고용량(4 x 10e6 CFU)으로 경구 투여하면 메타콜린에 대한 기도 반응이 감소하며, Der p 민감성 및 항진성 쥐의 BAL액 내 TNF-α 수준, 흉선과 활성화 조절 케모카인(TARC; thymus and activation-regulated chemokine) 및 IL-17A을 감소시킴을 보였다. 또한, L. gasseri는 L. gasseri 투여를 받지 않은 Der p 민감성 및 항진성 쥐보다 L. gasseri 를 투여 받은 쥐에게서 성장 인자-α, IL-6 자극 비장 세포, 폐포대식세포를 생성하는 IL-17 세포의 수를 변형시키는 IL-17A의 생산을 감소시켰다. 결론적으로 L. gasseri의 경구 투여는알레르기성 천식의 마우스 모델에서 알레르겐 유발 기도 염증 및 IL-17 전 염증 면역 반응의 주요 특성을약화시킬 수 있으며, 이는 알레르기성 천식의 예방 또는 치료 가능성에 임상적 의미를 가질 수 있다.

Keywords

: #LactobacillusGasseri #L.gasseri #AllergicAsthma #Th17#Mite #Allergen #AllergenChallenge #MiteAllergen #DermatophoidesPteronyssinus #Derp#Microbiome #Microorganisms #Microbes#GutMicrobiome #GutMicrobiota #Probiotics

#락토바실러스가세리 #L.가세리 #알레르기성천식 #진드기#진드기알레르겐 #알레르기항원 #알레르겐항진 #데르마토피데스페로니스시누스 #마이크로바이옴 #장내미생물 #장내미생물총 #장내미생물군 #프로바이오틱스 #유산균

[출처: Jan, R.-L., Yeh, K.-C., Hsieh, M.-H., Lin, Y.-L., Kao, H.-F., Li,P.-H., … Wang, J.-Y. (2011). Lactobacillus gasseri suppresses Th17pro-inflammatory response and attenuates allergen-induced airway inflammationin a mouse model of allergic asthma. British Journal of Nutrition, 108(01),130–139.]

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[Lactobacillus gasseri] Lactobacillus gasseri suppresses Th17 pro-inflammatory response and attenuates allergen-induced airway inflammation in a mouse model of allergic asthma

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