Identification of Lactobacillus plantarum genes modulating the cytokine response of human peripheral blood mononuclear cells
Saskia van Hemert 1,2 , Marjolein Meijerink 1,3 , Douwe Molenaar 1,2,4 , Peter A Bron 1,2,5 , Paul de Vos 1,6 , Michiel Kleerebezem 1,2,7 ,
Jerry M Wells 1,3 , Maria L Marco 1,2,8*
Abstract
Modulation of the immune system is one of the most plausible mechanisms underlying the beneficial effects of probiotic bacteria on human health. Presently, the specific probiotic cell products responsible for immunomodulation are largely unknown. In this study, the genetic and phenotypic diversity of strains of the Lactobacillus plantarum species were investigated to identify genes of L. plantarum with the potential to influence the amounts of cytokines interleukin 10 (IL-10) and IL-12 and the ratio of IL-10/IL-12 produced by peripheral blood mononuclear cells (PBMCs).
A total of 42 Lactobacillus plantarum strains isolated from diverse environmental and human sources were evaluated for their capacity to stimulate cytokine production in PBMCs. The L. plantarum strains induced the secretion of the anti-inflammatory cytokine IL-10 over an average 14-fold range and secretion of the proinflammatory cytokine IL-12 over an average 16-fold range. Comparisons of the strain-specific cytokine responses of PBMCs to comparative genome hybridization profiles obtained with L. plantarum WCFS1 DNA microarrays (also termed gene-trait matching) resulted in the identification of 6 candidate genetic loci with immunomodulatory capacities. These loci included genes encoding an N-acetyl-glucosamine/galactosamine phosphotransferase system, the LamBDCA quorum sensing system, and components of the plantaricin (bacteriocin) biosynthesis and transport pathway. Deletion of these genes in L. plantarum WCFS1 resulted in growth phase-dependent changes in the PBMC IL-10 and IL-12 cytokine profiles compared with wild-type cells.
The altered PBMC cytokine profiles obtained with the L. plantarum WCFS1 mutants were in good agreement with the predictions made by gene-trait matching for the 42 L. plantarum strains. This study therefore resulted in the identification of genes present in certain strains of L. plantarum which might be responsible for the stimulation of anti- or pro-inflammatory immune responses in the gut. |
제목
Identification of Lactobacillus plantarum genes modulating the cytokine response of human peripheral blood mononuclear cells.
내용
IL-10/IL-12를 말초 혈액 단핵 세포 (PBMCs)에 의해 생성되는 Lactobacillus plantarum의 유전자를 확인하기 위하여 유전적 및 표현형 다양성을 조사 하였다. 다양한 환경 및 인간 공급원으로부터 분리 된 총 42종의 유산균 (Lactobacillus plantarum) 균주를 PBMC에서 사이토카인 생성을 자극하는 능력에 대해 평가 하였다. L.plantarum 균주는 항 염증성 사이토카인 IL-10의 분비를 평균 14배 범위 이상으로 유도하고 염증성 사이토카인 IL-12의 분비를 평균 16배 범위 이상으로 유도했다.
[출처 : van Hemert et al. BMC Microbiology 2010, 10:293]
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