[Lactobacillus paracasei] Decreased Fat Storage by Lactobacillus Paracasei Is Associated with Increased Levels of Angiopoietin-Like 4 Protein (ANGPTL4)

   Abstract

   Background:

  Intervention strategies for obesity are global issues that require immediate attention. One approach is to exploit the growing 

  consensus that beneficial gut microbiota could be of use in intervention regimes. Our objective was to determine the mechanism by 

  which the probiotic bacteria Lactobacillus paracasei ssp paracasei F19 (F19) could alter fat storage. Angiopoietin-like 4 (ANGPTL4)

  is a circulating lipoprotein lipase (LPL) inhibitor that controls triglyceride deposition into adipocytes and has been reported to be

  regulated by gut microbes.

   Methodology / PrincipalFindings :

  A diet intervention study of mice fed high-fat chow supplemented with F19 was carried out to study potential mechanistic   effects on

  fat storage. Mice given F19 displayed significantly less body fat, as assessed by magnetic resonance imaging,  and a changed 

  lipoprotein profile. Given that previous studies on fat storage have identified ANGPTL4 as an effector, we also investigated

  circulating levels of ANGPTL4, which proved to be higher in the F19-treated group. This increase, together with total body fat and

  triglyceride levels told a story of inhibited LPL action through ANGPTL4 leading to decreased fat storage. Co-culture experiments of

  colonic cell lines and F19 were set up in order to monitor any ensuing alterations in ANGPTL4 expression by qPCR. We observed 

  that potentially secreted factors from F19 can induce ANGPTL4 gene expression, acting in part through the peroxisome proliferator

  activated receptors alpha and gamma. To prove validity of in vitro findings, germ-free mice were monocolonized with F19. Here we

  again found changes in serum triglycerides as well as ANGPTL4 in response to F19.

   Conclusions/Significance:

  Our results provide an interesting mechanism whereby modifying ANGPTL4, a central player in fat storage regulation, through 

  manipulating gut flora could be an important gateway upon which intervention trials of weight management can   be based.

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[출처 : PLoS ONE September 2010 | Volume 5 | Issue 9 | e13087 ]